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. 2019 Jul 31;10:1826. doi: 10.3389/fimmu.2019.01826

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Copyright © 2019 Huang, Zhang, Xu, Tang, Li, Wang, Liu and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression profiles of CgIKKα/β-2 mRNA in different tissues (A) and following challenge with different stimulants (B–D) as determined by qRT-PCR. Tissues: GO, Gonad; MU, Muscle; BL, Blood; MA, Mantle; GI, Gill; LA, Labial palp; DI, Digestive gland. β-actin gene expression was used as an internal control, and gonad was used as a reference sample. Vertical bars represent the mean ± SD (N = 3). Challenges: the different stimulants is LPS, PGN, and poly(I:C). The expression level of CgIKKα/β-2 was determined at 0, 3, 6, 12, 24, 48, and 72 h after challenge. β-actin gene expression was used as an internal control, and time 0 h was used as a reference sample. Vertical bars represent the mean ± SD (N = 3).