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. 2019 Jul 31;10:1826. doi: 10.3389/fimmu.2019.01826

Figure 6.

Figure 6

Interactions between oyster IKKα/βs and other proteins detected by co-immunoprecipitation (Co-IP) and yeast two hybrid (Y2H) assays. (A) Co-IP results showing that CgMyD88-1 directly interacts with CgIKKα/β-2 but not with CgIKKα/β-1. Asterisk represents the heavy chain of mouse IgG. Anti-Myc western blot bands show the expression of CgMyD88-1-Myc and anti-FLAG western blot bands show the expression of CgIKKα/β-1-FLAG and CgIKKα/β-2-FLAG. (B) Interaction between CgIKKα/β-2 and CgMyD88-1 was detected by Y2H assay. DDO, -Leu/-Trp double-dropout media; QDO/X/A, -Ade/-His/-Leu/-Trp quadruple dropout media with X-α-Gal and aureobasidin A. pGADT7-T and pGBKT7-53 were used for the positive control; pGADT7-T and pGBKT7-Lam were used for the negative control. (C) Co-IP results showing that CgTRAF6 directly interacts with CgIKKα/β-1 and CgIKKα/β-2. Anti-Myc western blot bands show the expression of CgTRAF6-Myc and anti-FLAG western blot bands show the expression of CgIKKα/β-1-FLAG and CgIKKα/β-2-FLAG. (D) Interaction between CgIKKα/β-2 and CgTRAF6 was detected by Y2H assay.