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. 2019 Jul 31;10:979. doi: 10.3389/fpls.2019.00979

FIGURE 2.

FIGURE 2

Impaired SA signaling does not affect EMR triggered by Fo47. (A) Ten days old tomato seedlings (Moneymaker) and transgenic NahG plants impaired in SA accumulation were inoculated with water (mock), Fo47, Fol4287 or co-inoculated with Fo47 and Fol4287. Disease development was assessed by measuring fresh weight (B) and disease index (C) 3 weeks after inoculation. Raw data was analyzed by a non-parametric Mann–Whitney U-test (∗∗∗P < 0.001). The bioassay was performed three times with similar results, where the number of plants (n) was n = 10 for the first repetition and n = 20 for the second and third repetition. (D) Representative stem pieces harvested from crown (left) and cotyledon (right) level of five individual plants incubated for 4 days on PDA plates. Percentage of Fo47 colonization on mock-inoculated plants (n = 10) and Fo47-treated plants (n = 20). The experiment was performed three times with similar results. Raw data was analyzed by a non-parametric Mann–Whitney U-test (∗∗∗P < 0.001).