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. 2019 Jul 31;10:979. doi: 10.3389/fpls.2019.00979

FIGURE 5.

FIGURE 5

Impaired ethylene perception in tomato does not affect EMR triggered by Fo47. (A) Ten days old wild-type tomato seedlings (Pearson) and Nr plants impaired in ET perception were inoculated with water (mock), Fo47, Fol4287 or co-inoculated with Fo47 and Fol4287. Disease development was assessed by measuring fresh weight (B) and disease index (C) 3 weeks after inoculation. Raw data was analyzed by a non-parametric Mann–Whitney U-test (∗∗∗P < 0.001). The bioassay was performed twice with similar results [where the number of plants (n) was n = 10 for the first repetition and n = 20 for the second repetition]. (D) Representative stem pieces taken from crown (left) and cotyledon (right) level of five individual plants incubated for 4 days on PDA plates. Percentage of Fo47 colonization on mock-inoculated plants (n = 10) and Fo47 treated plants (n = 20). The experiment was performed twice with similar results. Raw data was analyzed by a non-parametric Mann–Whitney U-test (∗∗∗P < 0.001).