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. Author manuscript; available in PMC: 2020 May 15.
Published in final edited form as: Dev Biol. 2019 Mar 1;449(2):99–106. doi: 10.1016/j.ydbio.2019.02.015

Figure 3. hNCs induced by short pulse of CHIR retain potential for terminal differentiation.

Figure 3

hNCs induced on day 5 following either 2D CHIR or 5D CHIR treatment were subject to terminal differentiation with distinct protocols ranging from 2–6 weeks and assessed histologically by alizarin red for osteoblasts, alcian blue for chondrocytes, and oil red for adipocytes. Smooth muscle was evaluated by Immunofluorescence for smooth muscle actin, SMA (green); peripheral neurons for Peripherin (green) and HuC/D (red); glia by S100B (green), and Melanoblasts by SOX10 (green) and MITF (red).