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. Author manuscript; available in PMC: 2020 May 15.
Published in final edited form as: Dev Biol. 2019 Mar 1;449(2):99–106. doi: 10.1016/j.ydbio.2019.02.015

Figure 4. hNCs can be generated via 2D-CHIR in platforms of various dimensions.

Figure 4

SOX10 (green), and PAX7 (red) immunofluorescence of day 5 cultures treated with 2D 3uM CHIR on days 0–2. Images are from 96-well, 48-well, 24-well, 12-well, 6-well and 10cm dish formats. DAPI counterstain is grey on left column and blue in right merged column.