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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: Anal Biochem. 2019 Feb 27;572:1–8. doi: 10.1016/j.ab.2019.02.019

Fig. 1.

Fig. 1.

Separation of the NAD+ metabolites in standard solution using an Accucore HILIC column (2.1 × 150 mm, 2.6 μm, Thermo) at 32 °C with a chromatographic run time of 14 min. Mobile phase A consisted of 7.5 mM ammonium acetate with 1.54 mM ammonium hydroxide and mobile phase B was acetonitrile. The chromatogram is presented with intensity in cps (y-axis) and time (min) on the x-axis. The inset represents an expanded view of the peaks observed below 1.0e5 (y-axis) in a similar timescale.