Figure 2.

In vitro investigations of lignin NCs. Pch and Pmi were used as ligninolytic enzyme‐segregating fungi, whereas Eul was applied as control fungus without laccase segregation. A) Optical density (at 600 nm) of fungal cultures in 96‐well microtiter plates after 48 h for samples empty and pyraclostrobin‐loaded lignin NCs (see Table S1 for details, Supporting Information). B) HPLC‐MS analysis of NC supernatant after the incubation with culture filtrate of Pch after 24 and 72 h. Release compared to a pyraclostrobin standard curve and the amount of applied fungicide.