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. 2019 May 24;6(15):1900295. doi: 10.1002/advs.201900295

Figure 5.

Figure 5

Ccar1 and Rps14 are key target genes of IMP2 that mediate early embryonic developmental potential. A) Luciferase reporter activity of the indicated downregulated genes containing IMP2 binding sites. RLA, relative luciferase activity. Error bars indicate the SEM. B,C) Luciferase reporter activity of hCCAR1 in response to IMP2 in a dose‐dependent manner (B), and luciferase reporter activity of downstream mCcar1 in response to IMP2 (C). Error bars indicate the SEM. At least three independent experiments were performed for mean of each value. D) Schematic diagram showing the microinjection of early mouse zygotes and subsequent embryo analysis at the molecular and developmental level. E) Blastocyst development is defective after injecting siRNAs targeting the Ccar1 and Rps14 genes at the indicated times compared with control siRNA. Scale bar, 100 µm. F) Quantification of morula (56 h) and blastocyst (80 h) formation after injecting control siRNA or siRNAs targeting Ccar1 and Rps14. The numbers of embryos (n) analyzed are indicated. Error bars indicate the SEM. **p < 0.01, Student's t‐test. G) qRT‐PCR results showing the expression of IMP2 target genes in 2‐cell‐stage embryos after Ccar1/Rps14 deletion in zygotes. Error bars indicate the SEM. *p < 0.05, Student's t‐test.