Figure 3.

Suppression of UniCAR-equipped Tconvs by autologous TCR-activated Tregs. 0.5 × 10⁴ eFluor670-labeled, UniCAR-endowed Tconvs were cocultured with PC3-PSCA cells (effector to target cell ratio of 5:1) in the absence or presence of 6 pmol αPSCA TM. For immunosuppression, autologous, eFluor450-stained Tregs, which were either non-activated or pre-stimulated with αCD3/CD28 for 24 h prior to the assay, were added at indicated Treg:Tconv ratios. After four days, absolute cell number of eFluor670-labeled Tconvs was determined by flow cytometry and percent of suppression was calculated. Summarized data of three independent donors are depicted as mean ± SEM in the presence of (a) non- or (a,b) TCR-activated Tregs. Statistical significance was calculated by using (a) unpaired, two-tailed Student’s t-test or (b) 1-way ANOVA with Bonferroni post hoc test (*p < .05, **p < .01, ***p < .001).