Fig. 2.

Activation of p53 in INS-1 832/13 cells, rodent islets and human islets upon exposure to HG conditions and in ZDF pancreatic islets. INS-1 832/13 cells and normal rat islets (a) were incubated with LG (2.5 mM) or HG (20 mM) for 24 h and lysates were analyzed by western blotting for total and phosphorylated p53. Phospho-p53 band intensities were quantified by densitometry and ratios were calculated over total-p53 in INS-1 832/13 cells (b). *p < 0.05 vs. 2.5 mM glucose alone (n = 3). Phospho-p53 band intensities were quantified by densitometry and ratios were calculated over total-p53 in rodent islets (c). *p < 0.05 vs. 2.5 mM glucose alone (n = 3). Total and phosphorylated p53 levels were determined in human islets (d) following exposure to LG or HG as indicated. Total and phosphorylated p53 levels were determined in islet lysates from ZDF and ZLC rats (e) as indicated