Figure 2.

Effects of amiodarone (AMD) and itraconazole (ICZ) on infection of human-induced pluripotent cell–derived cardiomyocytes with TcII. Human-induced pluripotent cell–derived cardiomyocytes (2 × 10⁵/well) were infected with TcII at a ratio of five trypanosomes/cell for 24 hours at 37°C at 5% CO₂ and 80% humidity. Infections took place in the presence of amphotericin B (250 ng/mL), benznidazole (BNZ) (11.5 or 23 μM), ICZ (1.5, 4.5, or 7.5 μg/mL), AMD (2, 4, or 8 μM), or ICZ + AMD combinations. Infection rates (A) and multiplication (B) were determined 48 hours after infection. Statistics: Welch’s t-test. Comparisons without brackets: asterisks, RPMI vs. all other bars; squares, BNZ (23 μM) vs. all other bars. Other comparisons as indicated by the ends of the brackets. One, two, or three asterisks or squares = P ≤ 0.05, P ≤ 0.01, or P ≤ 0.001, respectively.