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. 2019 Jul 30;55(3):671–683. doi: 10.3892/ijo.2019.4852

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Copyright: © Wu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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miR-501-3p regulates colorectal cancer cell proliferation, cell cycle progression and sphere formation. (A) Transfection with miR-501-3p inhibitor decreased miR-501-3p expression levels in HCT116 and Caco2 cells. (B) The cell proliferation ability was inhibited following miR-501-3p downregulation in HCT116 cells. (C) The cell proliferation ability was inhibited following miR-501-3p downregulation in Caco2 cells. (D) Representative images of cell cycle distribution analysis in HCT116 and Caco2 cells transfected with miR-NC inhibitor or miR-501-3p inhibitor. (E) Quantification of cell cycle distribution analysis revealed that miR-501-3p downregulation accumulated cells in the G1 phase. (F) Representative images of sphere formation in HCT116 and Caco2 cells transfected with miR-NC inhibitor or miR-501-3p inhibitor. Scale bar, 20 µm. (G) Quantification of sphere formation assay revealed that miR-501-3p downregulation reduced the sphere formation ability of HCT116 and Caco2 cells. ^**P<0.01 and ^***P<0.001, with comparisons indicated by lines. miR, microRNA; NC, negative control.

miR-501-3p regulates colorectal cancer cell proliferation, cell cycle progression and sphere formation. (A) Transfection with miR-501-3p inhibitor decreased miR-501-3p expression levels in HCT116 and Caco2 cells. (B) The cell proliferation ability was inhibited following miR-501-3p downregulation in HCT116 cells. (C) The cell proliferation ability was inhibited following miR-501-3p downregulation in Caco2 cells. (D) Representative images of cell cycle distribution analysis in HCT116 and Caco2 cells transfected with miR-NC inhibitor or miR-501-3p inhibitor. (E) Quantification of cell cycle distribution analysis revealed that miR-501-3p downregulation accumulated cells in the G1 phase. (F) Representative images of sphere formation in HCT116 and Caco2 cells transfected with miR-NC inhibitor or miR-501-3p inhibitor. Scale bar, 20 µm. (G) Quantification of sphere formation assay revealed that miR-501-3p downregulation reduced the sphere formation ability of HCT116 and Caco2 cells. ^**P<0.01 and ^***P<0.001, with comparisons indicated by lines. miR, microRNA; NC, negative control.