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. 2019 Jul 11;8(9):e1629259. doi: 10.1080/2162402X.2019.1629259

Figure 1.

Figure 1.

E7 RNA-LPX immunization induces a strong antigen-specific effector and memory CD8+ T cell response in mice (a-d) Mice (n=5/group) were immunized twice with E7 RNA-LPX or NaCl (control) (d0, d7) and blood samples were harvested six days after each immunization (d6, d13). (a) Serum cytokines 3 h after first immunization were determined by multiplex immunoassay. (b-d) Flow cytometric characterization of de novo primed E749-57 MHC-class I multimer positive CD8+ T cells in the blood with regard to (b) their frequency and (c, d) their phenotype on d13. (c) Differential expression of CD127 and KLRG1 on E749-57 MHC-class I multimer positive (pos) and negative (neg) CD8+ T cells of E7 RNA-LPX immunized mice. (d) Differential expression of CD62L within KLRG1CD127+ (MPEC) E749-57-specific CD8+ T cells. (b, c) Representative pseudocolor plots are shown. Significance was determined using (a, b) unpaired, two-tailed Student’s t-test and (c) two-way ANOVA, Bonferroni post-test. Mean±SD. SLEC: Short-lived effector cells; MPEC: Memory precursor effector cells; Tem: T effector memory cells; Tcm: T central memory cells.