Fig 1. TgFBXO1 interacts with TgSKP1.
(A). RHΔhxgprtΔKu80 and TgSKP1SF tachyzoites were solubilized and immunoprecipitated with affinity purified anti-TgSKP1 (UOK75) or anti-FLAG (M2), respectively. The immunoprecipitates were trypsin digested and subjected to proteomic analysis. Protein candidates were selected from a list of protein hits that were detected in multiple replicates and minimally recovered with irrelevant antibodies (for UOK75) or the untagged strain (for M2), and are reported as found in either both (red bars) or only individual immunoprecipitations with either high or medium confidence. Proteins known or predicted to interact with TgSKP1 are underlined. See S2 Fig and S2 Table for origin of protein labels and more information about control data. (B). Lysates prepared from TgFBXO1HA and parental RHΔhxgprtΔKu80 grown at 21% or 0.5% O2 were either Western blotted (Input) or incubated with anti-HA antibodies (IP). The immunoprecipitates were captured by Protein G Sepharose and the immune complexes were Western blotted to detect TgFBXO1HA, TgSkp1, and SAG1.