Skip to main content
. 2019 Jul 26;15(7):e1007946. doi: 10.1371/journal.ppat.1007946

Fig 2. TgFBXO1 is important for Toxoplasma growth.

Fig 2

(A). Schematic illustration of HA(ATC)TgFBXO1 anhydrotetracycline (ATC)-regulated gene expression. The TgFBXO1 endogenous promoter was replaced with a SAG4 promoter containing a tetracycline transactivator (tTA) binding element. In the absence of ATC, the tetracycline regulated promoter is active, while the addition of ATC reduces transcription by preventing tTA from binding to and activating the promoter. (B). Lysates from HA(ATC)TgFBXO1 or parental TATiΔKu80 parasites grown for 24 h in the absence or presence of 1μg/ml ATC were Western blotted to detect HA(ATC)TgFBXO1 or SAG1 as a loading control. (C). HA(ATC)TgFBXO1 or TATiΔKu80 parasites (100/well of a 6 well plate) were grown for 5 d on HFF monolayers with or without 1μg/ml ATC. The cells were then fixed and numbers of plaques formed counted. Shown are the averages and standard deviations of 3 independent experiments performed in triplicate. (D). Representative images of plaques from (C) showing decreased plaque size of TgFBXO1-depleted parasites. Plaque sizes were quantified and plotted on the adjacent graph. *, p < 0.001, One-Way ANOVA. Bars = 0.5mm.