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. 2019 Jul 26;15(7):e1007946. doi: 10.1371/journal.ppat.1007946

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© 2019 Baptista et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — (A). TgFBXO1^HA-expressing parasites were fixed and stained to detect IMC3, TgFBXO1, and DNA during G1, S, and M phases. Arrows highlight TgFBXO1 apical structures. (B). TgFBXO1^HA-expressing parasites were fixed and stained to detect ISP1, TgFBXO1, and DNA during S, early M (before nuclear segregation), and late M (during nuclear segregation) phases. Arrows highlight TgFBXO1 apical structures. Bars = 2μm. (C). TOP: Fractionation scheme to characterize TgFBXO1^HA association with the IMC. BOTTOM: Western blotting of equivalent volumes of each indicated fraction. Blots were probed with antibodies to detect TgFBXO1 (α-HA), IMC3 (αIMC3), and ISP1 (αISP1).