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. 2019 Aug 7;14(8):e0220636. doi: 10.1371/journal.pone.0220636

Fig 11. The effectiveness of migration of gastric epithelial cells and fibroblasts exposed to H. pylori components.

Fig 11

The migration of primary gastric epithelial cells (A) and fibroblasts (B) unstimulated or treated with: glycine acid extract (GE) (10 μg/ml), cytotoxin associated gene A (CagA) protein (1 μg/ml), urease A subunit (Ure A) (5 μg/ml), H. pylori (Hp) or E. coli (Ec) lipopolysaccharide (LPS) (25 ng/ml) and detected in a scratch assay. (i) The migration of primary epithelial cells and fibroblasts shown as a graph presenting wound confluence [%] (average wound size against time). The results are presented as median values of four independent experiments performed in triplicates for each experimental variant. Statistical analysis was performed in the nonparametric U Mann-Whitney test with significance for *P < 0.05, **P < 0.01, ***P < 0.001 obtained for unstimulated cells vs cells treated with H. pylori antigens (according to the used stimulators). (ii) Phase-contrast microscopy images of a scratch assay. Pictures were taken at the indicated time points and the extent of wound closure for each treatment variant is shown (red arrows). Representative images of each time point for both types of cells are shown.