Figure 6.

Expression of 67-kDa Laminin Receptor and 2,3-Linked Sialic Acid, and mRNA Levels of scAAV Receptors, in the Control and OHT Eyes

(A–F) Expression of 67-kDa laminin receptor, a receptor for AAV8, was markedly enhanced in the ciliary body (A), iris (B), and corneal endothelium (C) of OHT compared with control eyes (D–F) but was similar in the TM between OHT and control eyes (A and D). Immunostaining with MAL II revealed upregulated expression of 2,3-linked sialic acid in the TM, ciliary body (G and J), iris (H and K), and corneal endothelium (I and L) of OHT eyes (G–L). qRT-PCR analysis showed that the mRNA levels of perlecan-1, syndecan-4, and 67-kDa laminin receptor were significantly elevated in the anterior segment of OHT compared with control eyes (M). Immunohistochemistry images are representatives of 45 mice analyzed (30 for OHT group and 15 for control). Scale bars, 20 μm. Data are means ± SEM.