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. 2019 Apr 25;70(15):3851–3866. doi: 10.1093/jxb/erz192

Fig. 3.

Fig. 3.

Grain phenotype of transgenic plants and LGS1-RNAi plants. (A–C) Grain phenotype of Ma85 and four transgenic lines of Ma85 expressing the genetic complementation vector pLGS1::LGS1 (A), JF178 and two lines of JF178 expressing plgs1::lgs1 (B), and Samba and JF178 expressing the LGS1-RNAi construct (C). Scale bars=1 cm in (A–C). (D–F) Transcript levels of LGS1 in Ma85 and four transgenic lines of Ma85 expressing pLGS1::LGS1 (D), JF178 and two transgenic lines of JF178 expressing plgs1::lgs1 (E), and Samba, JF178, and transgenic RNAi plants (F). RNA was isolated from young panicles (5 cm long, n=4). MaCP, transgenic Ma85 expressing the complementation vector pLGS1::LGS1; JFCP, transgenic JF178 expressing plgs1::lgs1. Ma85, JF178, and Samba and JF178 were used as controls in (A) and (D), (B) and (E), and (C) and (F), respectively. Values are given as the mean ±SE. ns P>0.05, **P<0.01 were calculated by Student’s t-test.