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. 2019 Apr 11;70(15):3969–3979. doi: 10.1093/jxb/erz166

Fig. 5.

Fig. 5.

Interactions between AaTGA3 and AaTGA6. (A) Yeast two-hybrid assays with AaTGA3 and AaTGA6 fused to GAL4 activation domain (AD) or GAL4-binding domain (BD). Interactions were examined on synthetic dropout media either without Leu and Trp (SD-TL), without His, Leu, and Trp (SD-TLH), or without Ade, Leu Trp, His but containing 80 mM 3 AT (SD-ALTH). 3 AT inhibited the self-transcription activity of AaTGA6-BD. (B) Interactions between AaTGA3 and AaTGA6 were examined by BiFC in N. benthamiana leaves. AaTGA3 and AaTGA6 were fused in the vectors pEarleyagte201 and pEarleyagte202, respectively. (C) Pull-down assay showing the interaction of AaTGA6 and AaTGA3. Prokaryotic expression of AaTGA6 and AaTGA3 proteins fused to His or a GST tag were subjected to a GST pull-down, and the proteins were detected by immunoblotting using a GST antibody.