Fig. 6.

AaTGA3 represses the DNA-binding activity of AaTGA6. (A) Schematic diagram of the reporter and effector constructs in the transient dual-LUC assays. (B) Transient dual-LUC analysis showing that AaTGA3 inhibits AaTGA6 activation of the transcription of AaERF1. Luc/Ren represents the luciferase/Renilla ratio of n=3 independent experiments; Significant differences were determined using Student’s t-test: *P<0.05; **P<0.01. (C) EMSA analysis showing that AaTGA3 inhibits the DNA-binding activity of AaTGA6.