Fig. 1.

Interaction between rice NF-YB1 and NF-YC12. (A) Yeast two-hybrid assay. The full-length and truncated NF-YC12 cDNAs were cloned into a vector bearing the DNA binding domain (BD), and the full length cDNAs of NF-YB1 were cloned into a vector bearing an activation domain (AD). The transformants were grown on DDO (SD/–Leu/–Trp), QDO (SD/–Leu/–Trp/–His/–Ade), and QDO with X-α-Gal plates. (B) BiFC assays of NF-YC12 and NF-YB1. NF-YB1-cCFP and NF-YC12-nCerulean interacted to form a functional CFP in rice protoplast cells. Scale bars are 5 μm. (C). Pull-down assays Showing that there was a direct interaction between GST-NF-YB1 and His-NF-YC12 in vitro. IB, immunoblotting.