Skip to main content
. 2019 Apr 30;70(15):3825–3833. doi: 10.1093/jxb/erz202

Fig. 1.

Fig. 1.

MinION sequencing without Ds enrichment. (A) A schematic diagram of Ds insertion in the soybean genome. The length of the Ds insertion is 1166 bp. The positions of forward (F) and reverse (R) primers used for PCR genotyping are shown. (B) Workflow of direct genome sequencing without target enrichment. Genomic DNA was end-repaired and dA-tailed, ligated with sequencing adaptors, and sequenced on the FLO-MIN106 flow cell. (C) A schematic diagram of the Ds insertion in the Glyma.15g128600 gene. Two reads are shown. The first one covers 2347 bp in the 5'-flanking region and 3047 bp in the 3'-flanking region. The second one contains 370 bp flanking sequence in the 3' region. (D) PCR validation of the Ds insertion in Line 1. Thorne was used as control plant. The length of the DNA fragment without the Ds element in control plant is 360 bp, while the fragment length from Ds-containing Line 1 is 1526 bp.