Fig. 3.

Sequencing results after one-round enrichment of the Ds-containing fragments. (A and B) Schematic diagram of the flanking sequences of Line 2 (A) and Line 3 (B). Partial sequences of reads are shown. (C) PCR validation of the Ds insertion in Lines 2 and 3. Thorne was used as the control plant. The lengths of the DNA fragment without the Ds element are 612 bp for Line 2 and 689 bp for Line 3. With the Ds elements, the lengths of the DNA fragments are 1778 bp for Line 2 and 1855 bp for Line 3.