Table 1.
Comparison of the Effects of Cariprazine and AY9944 on Sterol Levels in Neuro2a.a
| Cariprazine (nM) | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cariprazine | 0 (DMSO) | 0.1 | 0.5 | 1.0 | 5.0 | 10.0 | 25.0 | 50.0 |
| Cholesterol | 6.40 ± 0.39 | 7.26 ± 0.34 | 6.14 ± 0.15 | 6.05 ± 0.52 | 5.58 ± 0.41 | 3.69 ± 0.39 | 3.58 ± 0.31 | 3.62 ± 0.58 |
| 7-DHC | 0.07 ± 0.01 | 0.12 ± 0.02 | 0.19 ± 0.03 | 1.31 ± 0.14 | 4.80 ± 0.97 | 8.01 ± 0.89 | 7.41 0.60 | 7.68 ± 0.61 |
| 7-DHD | 0.13 ± 0.02 | 0.16 ± 0.04 | 0.25 ± 0.01 | 2.54 ± 0.51 | 7.50 ± 1.20 | 8.50 ± 0.41 | 8.29 ± 0.77 | 9.05 ± 0.80 |
| Desmosterol | 5.09 ± 0.60 | 5.22 ± 0.14 | 4.54 ± 0.07 | 3.50 ± 0.16 | 2.66 ± 0.55 | 1.57 ± 0.12 | 1.33 ± 0.09 | 1.51 ± 0.14 |
| Lanosterol | 0.04 ± 0.01 | 0.05 ± 0.01 | 0.04 ± 0.01 | 0.05 ± 0.01 | 0.06 ± 0.01 | 0.05 ± 0.01 | 0.05 ± 0.01 | 0.04 ± 0.01 |
| AY9944 (nM) | ||||||||
| AY9944 | 0 (DMSO) | 0.1 | 0.5 | 1.0 | 5.0 | l0.0 | 25.0 | 50.0 |
| Cholesterol | 6.40 ± 0.39 | 4.44 ± 0.35 | 4.32 ± 0.31 | 4.79 ± 0.50 | 4.51 ± 0.07 | 4.07 ± 1.10 | 3.33 ± 0.07 | 3.56 ± 0.23 |
| 7-DHC | 0.07 ± 0.01 | 0.08 ± 0.01 | 0.14 ± 0.05 | 1.09 ± 0.14 | 3.72 ± 0.63 | 8.48 ± 0.49 | 7.96 ± 1.86 | 9.35 ± 0.57 |
| 7-DHD | 0.13 ± 0.02 | 0.15 ± 0.02 | 0.15 ± 0.03 | 0.80 ± 0.12 | 4.35 ± 0.57 | 6.02 ± 0.58 | 6.05 ± 0.38 | 7.54 ± 0.44 |
| Desmosterol | 5.09 ± 0.60 | 1.12 ± 0.11 | 1.24 ± 0.10 | 1.30 ± 0.18 | 1.67 ± 0.16 | 1.49 ± 0.05 | 1.41 ± 0.03 | 1.56 ± 0.14 |
| Lanosterol | 0.04 ± 0.01 | 0.03 ± 0.01 | 0.02 ± 0.01 | 0.02 ± 0.01 | 0.04 ± 0.01 | 0.03 ± 0.01 | 0.03 ± 0.01 | 0.04 ± 0.01 |
a
Neuro2a cells were incubated for 48h in sterol free medium in the absence or presence of different nM concentrations of cariprazine and AY9944. Sterol values correspond to the mean ± SE of 3 replicates in nmol/million cells. Numbers marked in bold and italics are significantly different from controls.