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. Author manuscript; available in PMC: 2019 Aug 7.
Published in final edited form as: J Leukoc Biol. 2019 Feb 18;105(4):783–795. doi: 10.1002/JLB.4A1018-381R

FIGURE 3. HIV-1BaL induced TLR4 expression is critical for the response of HIV-1BaL-infected CD68+ macrophages to LPS stimulation.

FIGURE 3

(A) Flow cytometry analyses of CD14 and TLR4 expression (n = 15; P = 0.003 and P = 0.0007) directly before and after HIV-1BaL infection; (B) TLR4 mRNA (n = 8; P = 0.016) and MD2 mRNA (n = 8; P = 0.016) were measured by RT-PCR; (C) Primary isolated CD68+ liver macrophages were treated with medium, poly I/C (2.5ug/ml), or HIV-1BaL infection (MOI = 0.1) for 24 hr without ATP treatment and TLR4 expression compared with TLR4 fluorescent intensity and examined by immunostaining. TLR4 (red), CD68 + liver macrophages (green), gp120 (magenta), and nucleus (blue). (D) CD68+ macrophages were treated by 0.1 MOI HIV-1BaL (n = 7) and or LPS stimulation (n = 7, P = 0.016) with or without 3 μM TAK-242. IL-1β was measured by ELISA. Panel A,C: Paired t test; Panel B: Wilcoxon signed rank test; Panel D: Kruskal-Wallis one way analysis (HIV vs. HIV+TLR4 blocking: Paired t test; LPS+HIV vs. HIV+LPS+TLR4 blocking: Wilcoxon signed rank test)