Figure 1.

Stratified preT1D subjects parallel relationships found between control subjects and patients with T1D. (a) PBMCs from nonautoimmune control subjects and patients with long-standing T1D were stained to determine Th40-cell levels, total CD4 and CD8 levels, and CD45RO levels in the CD4lo and CD4hi and run-in flow cytometry. Cells were gated on FSC and SSC for live cells, then gates were set on the basis of isotype controls. (b) PreT1D subjects were stratified into groups as shown in Table 2, then subjects in groups 1 through 4 were compared with subjects in groups 5 and 6 by staining and gating as in (a). Significant differences reported in both sets of graphs were calculated by two-tailed t test.