Fig. 5. Foxp3 promotes functional recruitment of Bcl11b in T_reg cells.

(A) Genome-wide occupancy of Bcl11b in WT T_reg (T_r) and T_fn cells derived from Foxp3^GFPKO mice. Bcl11b binding within a window ±3 kb centered on peaks identified by ChIP-seq analyses (indicated as a yellow triangle). Normalized enrichments of indicated Bcl11b peak sets are shown on the right as histogram plots. (B) Examples of Bcl11b peaks in T_n, T_r, and T_fn cells as viewed in IGV, representing the indicated categories. Red arrows indicate peaks that are significantly reduced in T_fn cells compared to Tr. (C) Graphical representation summarizing the subcategories to which the Bcl11b peaks lost in T_fn cells belong. (D) CDF analysis of gene expression changes between Bcl11b-deficient versus Bcl11b-sufficient T_reg cells for indicated gene subsets. Two-tailed Kolmogorov-Smirnov test P values are shown.