KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti-LAMP2 | ThermoFisher | Cat# PA1–655 RRID:AB_2134625 |
| Mouse anti-rabbit monoclonal IgG-HRP | Santa Cruz Biotech | Cat# sc-2357 RRID:AB_2714189 |
| Rabbit polyclonal anti-MEF2A | ThermoFisher | Cat# PA5–27380 RRID:AB_2544856 |
| Chemicals, Peptides, Metabolites and Recombinant Proteins | ||
| [5,5,5-2H3] leucine tracer | Sigma Aldrich | Cat#486825–1G |
| Liquid meal | Ensure, Abbott Laboratories | N/A |
| L-Norleucine, ≥98% (TLC) | Sigma-Aldrich | Cat# N6877 |
| 2-Mercaptoethanol | Sigma-Aldrich | Cat# M6250 |
| 4X Laemmli Sample Buffer | Bio-Rad | Cat# 1610747 |
| MSD-Tris lysis buffer | Mesoscale Discovery | Cat# R60TX-3 |
| Halt™ Protease Inhibitor Cocktail (100X) | ThermoFisher | Cat# 78430 |
| Ponceau S | Fisher Scientific | Cat# BP103–10 |
| REVERT total protein stain | LiCOR | Cat# 926–11011 |
| RNALater | ThermoFisher | Cat# AM7020 |
| HPLC-grade acetonitrile | ThermoFisher | Cat#A955–500 |
| HPLC-grade methanol | ThermoFisher on | Cat#A456–500 |
| HPLC-grade water | ThermoFisher | Cat#W64 |
| MS-grade formic acid | Sigma-Aldrich | Cat#399388 |
| Calibration solution | Agilent Technologies | Cat#G1969–85000 |
| N-acetyl Aspartic acid-2H3 | Sigma-Aldrich | Cat#616060 |
| Tryptophan-15N2 | Sigma-Aldrich | Cat#574600 |
| Sarcosine-2H3 | Sigma-Aldrich | Cat#493120 |
| Glutamic acid-2H5 | Sigma-Aldrich | Cat#616281 |
| Thymine-2H4 | Sigma-Aldrich | Cat# 487066 |
| Gibberellic acid | Sigma-Aldrich | Cat# G7645 |
| Trans-Zeatine | Sigma-Aldrich | Cat# Z0876 |
| Anthranilic acid 15N | Cambridge isotopes | Cat#NLM-3294 |
| Testosterone-2H3 | Sigma-Aldrich | Cat# T2655 |
| Jasmonic acid | Sigma-Aldrich | Cat# J2500 |
| Critical Commercial Assays | ||
| Insulin Detection Assay | Siemens | Cat# LKIN1 |
| CAF ELISA kit | Neurotune | Cat# NT1001 |
| RNeasy Plus Universal Mini Kit | QIAGEN | Cat# 73404 |
| TaqMan Universal PCR Master Mix | ThermoFisher | Cat# 4304437 |
| SuperScript VILO Master Mix | ThermoFisher | Cat# 11755250 |
| Bradford Assay, Bio-Rad Assay Dye | Bio-Rad | Cat#5000006 |
| NanoString nCounter Customized Gene Expression Assay | NanoString Technologies | N/A |
| Pierce™ Rapid Gold BCA Protein Assay Kit | ThermoFisher | Cat# A53225 |
| Oligonucleotides | ||
| Taqman FAM Probe MEF2A | ThermoFisher | Hs01050406_g1 |
| Taqman FAM Probe CKMT2 | ThermoFisher | Hs00176502_m1 |
| Taqman VIC Probe B2M | ThermoFisher | Hs00187842_m1 |
| Taqman FAM Probe LAMP2 | ThermoFisher | Hs00174474_m1 |
| Software and Algorithms | ||
| nSolver analysis software | Nanostring Tech | N/A |
| Quant Studio Design and Analysis Software 1.3.1 | Thermofisher | N/A |
| Image Studio Lite v5.2 | LI-COR Biosciences | N/A |
| Sigma Plot Version 13 | Systat Software, Inc | N/A |
| EndNotes Version 8.2 | Clarivate Analytics | N/A |
| SAS Version 9.4 | Sas Institute Inc | N/A |
| Others | ||
| Amicon ultracel-3K Membrane; | Millipore Corporation, | Cat#UFC5003BK |
| Vacuum | Genevac EZ-2plus | Cat#EZ-2.3 |
| Data generated with Nanostring technology can be found in Data S1 at the following link https://data.mendeley.com/datasets/ry4dmc24n6/draft?a=075d9c6d-8b8a-4c0f-b140-ae88a46e04fc | ||