Table 3.
Comparison of amino acid sequences of Zap1, Sp1, and MTF- 1 versus ZNF658. Homologous ZF domains are found in all of these proteins. The AD1 domain of Zap1 has regulatory element-binding characteristics which may be analogous to those of ZNF658, though direct homology of the domains is indeterminate
| Subject organism | Subject protein | Subject domain (position) | Human ZNF658 homologous region | E value | Identity (%) | Positives (%) | Total bit score (BLOSUM62) |
|---|---|---|---|---|---|---|---|
| S. cerevisiae | Zap1 | (all) | – | – | – | – | 1649 |
| S. cerevisiae | Zap1 | AD1 (258–317) | 199–253 | 8.00E-03 | 26 | 35 | – |
| H. sapiens | Sp1 | (all) | – | – | – | – | 1072 |
| H. sapiens | Sp1 | Zn finger 1 (626–650) | 886–902 (ZF16) | 2.00E-07 | 76 | 82 | – |
| H. sapiens | Sp1 | Zn finger 2 (656–680) | 523–540(ZF3) | 4.00E-04 | 44 | 72 | – |
| H. sapiens | Sp1 | Zn finger 3 (686–708) | 440–462(ZF2) | 9.00E-06 | 43 | 69 | – |
| H. sapiens | Sp1 | NLS/Zn fingers 1–3 (626–708) | 971–1040(ZFs 19–21) | 3.00E-20 | 49 | 62 | – |
| H. sapiens | MTF-1 | (all) | – | – | – | – | 1705 |
| H. sapiens | MTF-1 | NLS/Zn fingers 1–3 (112–202) | 768–903 (ZFs 12–16) | 2.00E-31 | 44 | 58 | – |