(A) Representative western blot analysis of VPS35, VPS26b, VPS29 proteins in N2A-APPswe cells transfected with VPS35 plasmid (VPS35) or vector control (Ctrl). (B) Densitometry of the immunoreactivities shown in the previous panel (*p<0.05, n=6). (C) Representative images of immunofluorescence analysis of cells transiently transfected with VPS35 cDNA and incubated with primary antibody for VPS35 (green). (D) Aβ1‐40 levels in conditioned media from cell transfected with empty vector (Ctrl) or VPS35 plasmid (VPS35) were measured by sandwich enzyme‐linked immunosorbent assay (*p<0.05, Ctrl, n=6; VPS35, n=6). (E) Representative Western blots of amyloid precursor protein (APP), sAPPα and sAPPβ in N2A-APPswe cells transfected with VPS35 plasmid (VPS35) or vector control (Ctrl). (F) Densitometric analyses of the immunoreactivities to the antibodies shown in the previous panel. Values represent mean ± standard error of the mean (Ctrl: n=6; VPS35, n=6; *p<0.05).