Figure 1.

Plumbagin inhibits the proliferation of canine cancer cells. (a) Structural similarities between plumbagin and ubiquinone are indicated by the red box. (b) The canine cancer cell lines (CTAC, 17CM, Denny and Peyton) were treated with plumbagin (0–10 μM) for 48 and 72 hours and proliferation of the cell lines was determined using MTT assay. The average of eight wells was used to plot the bar graphs for each condition tested. Data shown is representative of three independent biological replicates. *p = 0.013, **p = 0.003, and ***p = 0.0002 of test versus controls for each time point.