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. 2019 Aug 7;9:11471. doi: 10.1038/s41598-019-47261-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ATP did not prevent NKA inhibition by plumbagin. (a) The time course plot for the control and plumbagin (5 μM) treated CTAC cell is shown using ATP (5 mM) supplemented pipette solution. (b) Voltage steps plot at +50 and –160 mV shows inhibition of NKA activity even in the presence of ATP in the pipet solution (trace color same as in 4B). (c) The normalized current-voltage plot for all three, control (black trace) and plumbagin-treated (red trace) CTAC cells are shown. The application of the voltage steps and current measurement are identical to those used in Fig. 4.