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. 2019 Aug 7;9:11433. doi: 10.1038/s41598-019-47389-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extracellular matrix (ECM) deposition at the distal end of limb stumps for all groups at different time points. (A) Representative images of Picro Sirius Red stained collagen network at the distal end of limb stumps for control, sham and EStim groups at 3, 7, and 28 days post amputation (Scale bar = 100 µm). (B) Graph showing lower anisotropy (parallelism between fibrils) in electrically stimulated stump tissue compared to non-treated stumps, at all the time points evaluated. (C) Graph showing distance between fibrils measured at days 7 and 28 post-amputation. The greatest distance between fibrils was shown in EStim treated tissue at day 28. *p ≤ 0.05.