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. 2019 Aug 7;9:11433. doi: 10.1038/s41598-019-47389-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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BrdU analysis of cell proliferation in the distal end of the stump in control, sham and EStim treated samples. (A) Top and middle rows represent overview of day 3 post-amputation control, sham and EStim stump sections stained with AB&OG and anti-BrdU antibody (red) counterstained with DAPI (blue) respectively (4×; Scale bar = 500 µm). Bottom row shows high magnification images of BrdU stainings (20×; Scale bar = 50 µm). (B) Representative images of stump sections from control, sham and EStim groups 7 days post-amputation. Top and middle rows show an overview of samples stained with AB&OG and anti-BrdU antibody (red) and counterstained with DAPI (blue) respectively (4×; Scale bar = 500 µm). Bottom row displays high magnification images (20×; Scale bar = 100 µm); (C) Ratio of proliferative cells measured at the distal end of the stump at days 3 and 7 post-amputation. n.s. - non significant (p ≥ 0.05); *p < 0.05.