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. 2019 Aug 7;9:11433. doi: 10.1038/s41598-019-47389-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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New vessel growth in the distal area of the EStim rat limb stumps 28 days post amputation. (A) Representative image displaying vascular ingrowth in the distal end of the EStim stump at day 28 post-amputation. (AB&OG staining; Scale bar = 100 µm). a – High magnification (40×; Scale bar = 10 µm) of new vessel. (B) Graph indicating density of new vessels at the distal end of the stumps in all 3 groups 7- and 28- days post amputation. n.s. - non significant (p ≥ 0.05).