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. 2019 Aug 7;9:11433. doi: 10.1038/s41598-019-47389-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

mRNA expression profiles in EStim treated limb stumps and non-treated controls (control and sham) are distinct. (A) Principle component analysis of normalized read counts showed strong effect of EStim treatment. Biological replicates for EStim (E1, E2, E3), sham (S1, S2, S3) and control (K1, K2, K3) samples are shown. (B) Top-10 significantly enriched biological processes in EStim and sham transcriptomes (FunReach analysis). Each the EStim and sham transcriptomes were tested against Rattus Norvegicus UniProt database, the top 10 most highly represented biological processes and the percentage of proteins predicted to belong to each category are shown for EStim (b1) and sham (b2) samples. The numbers shown at the right side of each bar indicated the % of genes (proteins) involved in each term and p-Value.