Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Aug 7;9:11468. doi: 10.1038/s41598-019-48003-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Effects of lumbosacral nerve root avulsion on the expression of Hsp27 protein in neurons of the anterior horn of the spinal cord. (a) Images of the procedure of lumbosacral nerve root avulsion injury in rats. Arrowheads indicate the injury site in the spinal cord (LNRA, lumbosacral nerve root avulsion; DR, dorsal root; DRG, dorsal root ganglion; VR, ventral root). (b) After the LNRA models of rats were established for 1, 3, 5, and 14 days, the Hsp27 protein level was detected by western blotting analysis in L4–L6 spinal cord tissue of the sham group (N = 4) and LNRA groups (N = 4). GAPDH was used as a loading control for western blotting. Hsp27 protein expression was quantified by densitometric analysis. (c) After LNRA injury for 3 days, Hsp27 (Green), NeuN (Red), and Dapi (Blue) were assessed by immunofluorescence staining in L4–L6 spinal cord tissue of the sham and LNRA groups. The representative image of neurons of the anterior horn of the spinal cord was shown. Scale bar, 50 μm. The results were expressed as fold change compared to the sham group. Each bar represents the mean ± SD. ^**P < 0.01 versus the sham group.