Figure 2.

Sankey chart depicting read and fast5 sequence file data flow analysis for Experiment B2. Because the target lambda DNA fragment was a subset of the overall lambda (background) sequence, no reads mapped exclusively to the target, and therefore all correctly mapped target reads appear in the “both” category at the 3-pronged terminal ends of each chart branch. Undecided read counts shown here include both reads that timed-out of the decision process (>2 seconds in the queue) and those that did not otherwise receive a decision.