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. 2019 Aug 7;9:11477. doi: 10.1038/s41598-019-47942-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The attachment rate to J2 of M. hapla in different concentrations of bacterial cell suspensions of the isolates i.10 and i.20 (Microbacterium spp.). J2 of M. hapla were incubated in 100 µl of different concentrations of the bacterial isolates i.10 (10⁶, 10⁵ and 10⁴ CFU) and i.20 (10⁵, 10⁴ and 10³ CFU) and the number of attached bacterial cells per nematode was determined. Error bars represent standard deviations (n = 3).