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. 2019 Aug 7;9:11473. doi: 10.1038/s41598-019-47913-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The expression of the p53A isoform at low levels restores the loss of fertility in the p53^5A-1-4 mutant after high-dose irradiation. The adult female flies of the indicated genotypes were irradiated at 0 or 40 Gy and incubated with non-irradiated males. The adults were transferred to new vials every 24 h. The fertility was scored by the number of pupae produced 7–8 days after the adults were removed. The data represent the mean ± SD of the number of pupae produced per day by a single female during the indicated period after irradiation of at least two independent experiments (ns p > 0.05, *p < 0.05, **p < 0.01). The transgenes were used as hemizygous (1×) or homozygous (2×) state. The fertility after irradiation at 10–15 days in each genotype compared to that of p53^5A-1-4 mutant is indicated below by setting the fertility of irradiated p53^5A-1-4 mutant as 1.