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. 2019 Aug 7;9:11485. doi: 10.1038/s41598-019-47991-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of Pc-INH on the NMR profiles of γ-CD in DMSO-d6. (A) Partial ¹H NMR spectra of γ-CD in different Pc-INH/γ-CD molar ratios (0:1 to 1:10), with numbers 1 to 6 indicating respective signals from γ-CD’s protons (1)–(6) (in reference with Fig. 1B). (B1) DOSY spectrum of γ-CD alone, exhibiting a set of proton signals aligned at the same diffusion coefficient (while the arrows indicate distinctive signals from the solvent). (B2) DOSY spectrum of γ-CD in the presence of Pc-INH (in Pc-INH/γ-CD molar ration of 1:2), showing the existence of two molecular species with distinctive diffusion coefficients: free and complexed γ-CD (complex).