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. 2019 Aug 7;9:11478. doi: 10.1038/s41598-019-47874-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of GPC-1 Inhibition on Prostate Cancer Cell Growth and Morphology. (A) Effect of GPC-1 shRNA (Genecopoeia, Rockville, MD) on expression of GPC-1 in PC-3 cells. (B) Effect of GPC-1 inhibition on cell morphology visualized by crystal violet staining at high seeding density. (C,D) Changes in cell growth in GPC-1 knockdown PC-3 cells visualized by crystal violet staining after 7 and 11 days of culture (C) followed by quantification (D) Data in (A,C,D) are representative of at least 3 separate experiments with at least 3 independent cell passages. Data in D are presented as the mean ± the S.E.M. of at least 3 (n = 3) separate passages. *Indicates a significant difference (p < 0.05) as compared to control as determined using a Student t-test.