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. 2019 Aug 7;9:11438. doi: 10.1038/s41598-019-47893-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Morphological characterization of ^CCD8Cas9 edited tomato lines. (a–i) WT- wild type plants with normal vegetative growth; ^CCD8Cas9 T1 transgenic lines of the ^CCD8Cas9-edited plants. (a–ii) Root morphology and (a–iii) plant height and branching of the wild type and ^CCD8Cas9 mutant. (a–iv) Comparison of tomato fruits obtained from the different ^CCD8Cas9 mutants in the T2 generation and wild-type plants. (b) Plant height after 3 month of growth, average ± SE (n = 11) (c) Quantitative estimate of secondary branches in 1 month old ^CCD8Cas9 mutated tomato plants, average ± SE (n = 7) (d) mature fruit size, average ± SE (n = 15) and (e) number of fruit produced average ± SE (n = 6), in ^CCD8Cas9 mutated lines and wild type plant. The different small letters not connected by same letter on each bar indicate statistically significant differences compared to wild-type plants (p-value < 0.05; Student’s t-test).