Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Aug 7;9:11438. doi: 10.1038/s41598-019-47893-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Resistance of tomato ^CCD8Cas9-edited T1 lines to the parasite infestation as compared to the wild type. To evaluate host resistance to the parasite, host roots of the tomato wild-type (WT) and ^CCD8Cas9 mutated T1 lines were rinsed after 3 months of infestation with P. aegyptiaca seeds. Tubercles larger than 2 mm in diameter were considered for analysis. (a) Number of P. aegyptiaca tubercles and shoots attached to the mutant and non-mutant tomato plants in the pot assay. Bars represent average of two experiments with four independent plants of each T1 line (irrespective of type of mutation, either homozygous or biallelic within the lines) ±SE values. (b) Representative images of parasite infestation of the host plant; WT shows number of parasites attached to the root while ^CCD8Cas9 mutants have reduced parasite infection. (c) Whole plant images of mutant lines 5 and 11 showing the parasite shoots and tubercles developed on those roots as compared to the WT plants. Red triangle indicates P. aegyptiaca shoots developed on host plant line 11, while host plant line 5 is free of P. aegyptiaca infestation.