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. 2019 Aug 7;9:11438. doi: 10.1038/s41598-019-47893-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Orobanchol content, genotyping and amino acid sequence analysis of the ^CCD8Cas9 mutated T1 plants. (a) Orobanchol contents in the roots of tomato ^CCD8Cas9 edited lines (1, 2, 5 and 11) as compared to wild type. LC-MS/MS analysis was done with two independent biological samples from each line. Data presented as average ± SE. (b) PCR product sequence alignment of ^CCD8Cas9 edited lines (1, 2, 5 and 11) used for LC–MS/MS analysis. PAM is shown in red; BsrI site shown in bold underlined; DNA deletions are shown in black dots and deletion sizes (nt) are marked on the right side of the sequences; Nucleotide sequence inside red box encode for stop codon. (c) Amino acid sequences for line 2a (His-243 deletion) and line 11a (His-243 and Pro-244 deletion) compared to wild-type (WT) CCD8 proteins.