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. 2019 Aug 7;9:11447. doi: 10.1038/s41598-019-47836-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Hyperglycaemia alters the energy metabolism of BMDMs. (A) Western blot membranes probed with anti-mouse AMPK, anti-mouse pS6 and anti-mouse β-actin primary antibodies. (B) Phospho-AMPK/β-actin ratio. (C) Phospho-S6/β-actin ratio. (D) Lactate release after 24 hours of incubation. (E) Oxygen consumption during the first hour of incubation. *p < 0.05 compared to normoglycaemic BMDMs, ⁺<0.05 compared with control glucose medium. The images of the gels were cropped, with full-length blots/gels presented in Supplementary Fig. S1. The samples were derived from the same experiment, and the gels/blots were processed in parallel. Data are presented as the means ± SEM (N of at least 3).