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. 2019 Aug 7;9:11494. doi: 10.1038/s41598-019-46818-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Evaluation of shorter anti-miRs targeting miR-140-3p. (a) Concentrations of free miR-140-3p (i.e., not bound to an LNA-anti-miR) at the end of the experiments, n = 3–22 EHTs per group. (b–e) Contractile force of Ctrl-EHTs, AE-EHTs and AE-EHTs pretreated with a long anti-miR-140-3p and four shorter anti-miR-140-3p (S1–S4). Bars show mean ± SEM, one-way ANOVA and Dunnett’s post-test for multiple comparisons against Ctrl, *p < 0.05, **p < 0.01, ***p < 0.001, all numbers refer to total number of EHTs per group.