FIG 6.

Overexpression of GntR phenocopies overexpression of sRNA-29 under conditions of growth on nitrite. P. denitrificans plus empty pLMB509 vector (solid line) or pLMB509 with gntR cloned into the overexpression site (dashed line) was grown under denitrification conditions with either 20 mM nitrate or 3 mM nitrite as the electron acceptor with 10 mM taurine added to induce GntR overexpression at time zero (indicated with an arrow). OD₆₀₀ (A), nitrite accumulation (B), and N₂O production (C) were measured under conditions of growth on nitrate, and OD₆₀₀ (D) and nitrite consumption (E) were measured under conditions of growth on nitrite. Error bars represent SD of results of comparisons between triplicate experiments, and where not visible, the bars were smaller than the symbols (P < 0.0001).